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LECSTER is the largest, open-access searchable database of lectin characterizations, clinical correlates and citations on the Internet.

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Copyright 2001-2011.

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Total number of records: 619 Matching records: 1

Pisum sativum : Garden pea

Molecular structure of
Garden pea:PSA, PsA

Pisum sativum

Lecster ID:532 Edit Entry
Source Organism:Garden pea
Species:Pisum sativum
Common Nomenclature:PSA, PsA
Class:Legume lectins
Index (PSA, PsA)
Characterization Notes:For Pisum isolectin characterization, see: Plant Mol Biol 1994 Jan;24(1):75-81 Pea lectin is a mixture of isolectins that can be separated by ion exchange chromatography. fucose residues were shown to be an importan determinant in the tight binding of glycopeptides to pea lectin. See: J Bio Chem 1981 (256); 6633-6640 For further characterization of monoclonal antibody 6,F-8 reacting with Lathyrus, Lens and Pisum lectins, see: FEBS Lett. 1989 Apr 10;247(1):77-80.
Biological Activity:agglutinates human group A erythrocytes at a titer of 1:4; group B erythrocytes at a titer of 1:4; group B erythrocytes at a titer of 1:2; by crude lectin preparation (16mg/ml). See: Int J Cancer 1970 (6):283 For characterization of mitogenic properties, see: J Biol Chem. 1974 Sep 25;249(18):6004-12 and Proc Nat Acad Sci 1973 (70); 3650-3654 The pea lectin is used in histochemistry for the staining of N-glycans and is extensively used to visualize the acrosomal status of spermatozoa. See: Human Reprod 1994 (9); 1322-1327 The lectin can be used for the purification of feline Tlymphocytes from periferal blood. See: J Immunol Meth 1994 (175); 189-199
Source Tissue:Seeds, roots
Specificity:PSA: Man/Glc(Man>Glc/GlcNAc)
Lectin 2: Man/Glc (d-mannose, d-glucose) The specificity of PSA is similar to Lens culinaris lectin and ConA.

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By Peter D'Adamo. Copyright 2001-2011.