Among these mitogens, only Pa-1 was mitogenic for both murine B-cells and T-cells, and the other mitogens were T-cell mitogens. Pa-2, the strongest T-cell mitogen.
Three mitogenic lectins, designated PL-A, PL-B, and PL-C, were purified from the roots of pokeweed (Phytolacca americana).Although all of three lectins have mitogenic activities, PL-B is a mitogenic lectin with the most potent hemagglutinating and mitogenic activities, and PL-C has almost no hemagglutinating activity.
Biological Activity:
Human B lymphocytes activated by pokeweed mitogen (PWM) undergo a proliferative response, differentiate into immunoglobulin producing cells (IPC), and release immunoglobulin into the supernatant fluid of the lymphocyte cultures. See: Ann Clin Lab Sci 1982 Nov-Dec;12(6):455-62 for further characterization, see: Biochemistry. 1974 Aug 27;13(18):3671-7
Pokeweed Antiviral Protein a novel cap-binding protein, PAP is a type I ribosome-inactivating protein (RIP) isolated from Phytolacca americana.
Several cap-binding proteins mediate processes such as pre-mRNA splicing, translation initiation, and mRNA turnover. PAP also cleaves supercoiled DNA into relaxed and linear forms.
B-cell precursor (BCP) leukemia represents one of the most radiation-resistant forms of human malignancy. PAP caused apoptosis of radiation-resistant primary BCP leukemia cells and killed greater than 99
