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The Blood Type Diet Archives Volume 4

Re: Indican Test, Indoles

Posted By: Joan
Date: May-19, 1998 at 00:02:28

In Response To: Indican Test/Lectin Test (Phil)

I am curious about this as well. Looking at the standard Medline literature did turn up an intriguing reference (below). Since the indole tested is already acidified, the use of formic acid has some resemblance to the chloroform step in Dr. D'Adamo's method of characterizing indole levels.

Clin Chim Acta 1980 Jan 31;100(3):231-237

A modified procedure for the determination of
5-hydroxyindoleacetic acid in the urine and cerebrospinal fluid
of patients with hepatic cirrhosis.

Dombro RS, Hutson DG

A method has been developed for the separation of 5-hydroxyindoleacetic acid from
substances in urine and cerebrospinal fluid which interfere with its fluorometric determination.
This involves the use of anion exchange column chromatography with elution of the
5-hydroxyindoleacetic acid by 3 mol/l formic acid in 60% methanol. The indole acid is then
assayed fluorometrically after reaction with o-phtalaldehyde. Urinary 5-hydroxyindoleacetic
acid values are reported for patients with stable cirrhosis and hepatic encephalopathy and
compared with those found in control subjects without liver disease.

Messages in This Thread

  • Indican Test/Lectin Test
    Phil -- Tuesday, 19 May 1998, at 7:21 a.m.
    • Re: Indican Test, Indoles
      Joan -- Tuesday, 19 May 1998, at 12:02 a.m.

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